PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

Psittacine beak and feather disease (PBFD) affects Psittaciformes worldwide. In Brazil, PBFD is an emerging disease that has threatened exotic and native Psittaciformes in captivity; however, reports in the country are still scarce. The aim of this study was to describe a natural outbreak of PBFD in rose-ringed parakeets (Psittacula krameri) from breeding facilities in the state of Ceará, Northeast of Brazil. Six fledgling parakeets presented progressive feather loss and apathy and were euthanized due to a presumptive diagnosis of PBFD. Samples from the organs were collected in 10% formalin. Feathers and fragments of the organs were frozen and later tested by polymerase chain reaction (PCR) using a conserved sequence of the viral genome, followed by sequencing and phylogenetic analysis. Grossly, multifocal to coalescent apteria were observed in rectrices and covering feathers. Microscopically, multiple areas showed a disordered increase of epithelial cells in the stratum germinativum of the skin's epithelial layer, as well as epithelial and follicular necrosis with botryoid inclusion bodies. In addition, cellular necrosis was noted in the thymus and bursa. All samples tested positive for beak and feather disease virus (BFDV) and were confirmed by sequence analysis. To the authors' knowledge, this is the first report of BFDV infection in the State of Ceará. Considering the risk of Psittacula krameri as a potential spreader of BFDV, we emphasize the need to establish control measures to prevent viral dissemination to neotropical psittacine birds

• PBFD avian circovirus parakeets ring-necked parakeet Brazil

Abstract in Portuguese:

A doença do bico e das penas dos psitacídeos (PBFD) afeta Psittaciformes em todo o mundo. No Brasil, PBFD é uma doença emergente que ameaça Psittaciformes exóticos e nativos em cativeiro; entretanto, os relatos no país ainda são escassos. O objetivo deste estudo foi descrever um surto natural de PBFD em periquitos-de-colar (Psittacula krameri) de criatórios no estado do Ceará, Nordeste do Brasil. Seis filhotes de periquitos apresentaram perda progressiva de penas e apatia, sendo eutanasiados devido o diagnóstico presuntivo de PBFD. Amostras dos órgãos foram colhidas em formalina a 10%, e penas e fragmentos dos órgãos foram congelados e posteriormente testados por PCR utilizando uma sequência conservada do genoma viral, seguida de sequenciamento e análise filogenética. Macroscopicamente, foram observados apterias multifocais a coalescentes nas penas retrizes e coberteiras. Microscopicamente, múltiplas áreas apresentaram aumento desordenado de células epiteliais no estrato germinativo da camada epidérmica da pele; assim como observou-se necrose epitelial e folicular com inclusões botrioides. Adicionalmente, notou-se necrose celular no timo e na bursa. Todas as amostras testaram positivo para o vírus da doença do bico e das penas (BFDV) e confirmado por sequenciamento. Para o conhecimento dos autores, este é o primeiro relato de infecção por BFDV no estado do Ceará. Considerando o risco de Psittacula krameri como potencial disseminador do BFDV, enfatizamos a necessidade de estabelecer medidas de controle para prevenir a disseminação viral para psitacídeos neotropicais.

• PBFD circovírus aviário periquito periquito-de-pescoço-anelado brasil

Abstract in English:

ABSTRACT.- Fecchio R.S., Gomes M.S., Kolososki J., Petri B.S.S., Rossi Jr J.L. & Gioso M.A. 2008. [Study of the occlusion biomechanics and adherence of acrylic resin (polimetilmetacrilate) in fractures of rhinotheca in toucans (Ramphastos toco).] Estudo da biomecânica oclusal e da aderência da resina acrílica auto-polimerizável (polimetilmetacrilato) em fraturas de rinoteca de tucanos (Ramphastos toco). Pesquisa Veterinária Brasileira 28(7):335-340. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8 superior, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: rfecchio@gmail.com Traction effort was applied perpendicularly to the axis of toucan beaks and for accomplishment of the essay a dynamometer was used. The beak was completely fractured when submitted to traction of 270.40 N. For fixation of the fractured beaks acrylic resin was applied using a strip of 2.0cm of width. The second beak presented resistance up to 69.75 N. The third beak was submitted to acid conditioning and resisted to force of 63.29 N. Other two new tests were accomplished the whole surface of the rhinotheca filled out. The fourth beak was not submitted to the acid attack and it resisted up to 134.40 N, and the fifth was submitted to the acid etch and it resisted up to 101.50 N. The present work did not observe statistical correlation and, consequently, differences among the procedures with previous use of acid attack and those without the use of the same.

• Toucans rhinotheca fracture beak tucano rinoteca bico Ramphastos toco polimetilmetacrilate polimetilmetacrilato fraturas

Abstract in Portuguese:

ABSTRACT.- Fecchio R.S., Gomes M.S., Kolososki J., Petri B.S.S., Rossi Jr J.L. & Gioso M.A. 2008. [Study of the occlusion biomechanics and adherence of acrylic resin (polimetilmetacrilate) in fractures of rhinotheca in toucans (Ramphastos toco).] Estudo da biomecânica oclusal e da aderência da resina acrílica auto-polimerizável (polimetilmetacrilato) em fraturas de rinoteca de tucanos (Ramphastos toco). Pesquisa Veterinária Brasileira 28(7):335-340. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8 superior, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: rfecchio@gmail.com Traction effort was applied perpendicularly to the axis of toucan beaks and for accomplishment of the essay a dynamometer was used. The beak was completely fractured when submitted to traction of 270.40 N. For fixation of the fractured beaks acrylic resin was applied using a strip of 2.0cm of width. The second beak presented resistance up to 69.75 N. The third beak was submitted to acid conditioning and resisted to force of 63.29 N. Other two new tests were accomplished the whole surface of the rhinotheca filled out. The fourth beak was not submitted to the acid attack and it resisted up to 134.40 N, and the fifth was submitted to the acid etch and it resisted up to 101.50 N. The present work did not observe statistical correlation and, consequently, differences among the procedures with previous use of acid attack and those without the use of the same.